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cell lysates  (Thermo Fisher)


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    Thermo Fisher cell lysates
    Cell Lysates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lysates/Triton+X-100/pm42259249-354-2-35
    Average 99 stars, based on 1 article reviews
    cell lysates - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    BIA-KA:

    Article Title: Design of multivalent pharmacological chaperones against Pompe disease via metal-free ligation.
    Article Snippet: .. Cell lysates were obtained directly in the well by adding 50 μl of 0.1% Triton X100, and stored for 48 h at −80 °C before protein quantification by BCA Assay (Pierce). .. GAA enzyme activity was determined on cell lysates in sodium acetate buffer pH 5.6 using 4-methylumbelliferyl-α-D-glucopyranoside (Sigma-Aldrich, St Louis, MO, USA) as substrate.

    Incubation:

    Article Title: Aerobic exercise alleviates allergic airway inflammation by suppressing circMETTL9 -mediated formation of macrophage extracellular traps
    Article Snippet: .. The cell lysates were then incubated with streptavidin-conjugated agarose magnetic beads (Cat#20164, Thermo Fisher Scientific, Waltham, MA, USA) at standard temperature. ..

    Article Title: MCUB Inhibits PRKN‐Dependent Mitophagic Degradation of PD‐L1 to Promote Immune Evasion in Bladder Cancer
    Article Snippet: .. Cell lysates (RIPA buffer, Thermo Fisher) were incubated overnight at 4 °C with anti‐MCUB (Abcam ab204259, 1:50), anti‐PRKN (Cell Signaling #4211, 1:50), or anti‐PD‐L1 (Abcam ab213524, 1:50) antibodies. .. Protein A/G magnetic beads (Thermo Fisher) were added and incubated at 4 °C for 2 h. Beads were washed four times, and immune complexes were analyzed by Western blotting.

    Article Title: MCUB Inhibits PRKN-Dependent Mitophagic Degradation of PD-L1 to Promote Immune Evasion in Bladder Cancer.
    Article Snippet: .. Cell lysates (RIPA buffer, Thermo Fisher) were incubated overnight at 4 °C with anti-MCUB (Abcam ab204259, 1:50), anti-PRKN (Cell Signaling #4211, 1:50), or anti-PD-L1 (Abcam ab213524, 1:50) antibodies. .. Protein A/Gmagnetic beads (Thermo Fisher) were added and incubated at 4 °C for 2 h. Beads were washed four times, and immune complexes were analyzed by Western blotting.

    Article Title: Cholangiocyte-derived exosomal miR-381-3p promotes hepatic stellate cell activation and cholestatic liver fibrosis via targeting Klf6
    Article Snippet: .. After 48 h, cell lysates were incubated with streptavidin-coated beads (Thermo Fisher) to capture the biotinylated miRNAs and their associated RNA-protein complexes. ..

    Magnetic Beads:

    Article Title: Aerobic exercise alleviates allergic airway inflammation by suppressing circMETTL9 -mediated formation of macrophage extracellular traps
    Article Snippet: .. The cell lysates were then incubated with streptavidin-conjugated agarose magnetic beads (Cat#20164, Thermo Fisher Scientific, Waltham, MA, USA) at standard temperature. ..

    Article Title: Tumor cells metabolically resist immune-checkpoint therapy by macrophage efferocytosis-mediated fatty acid recycling.
    Article Snippet: Fixed cells were lysed in sodium dodecyl sulfate (SDS) lysis buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA, and 1% SDS) and subjected to DNA fragmentation using the Covaris ME220. .. The fragmented cell lysates were diluted 10-fold with dilution buffer (10 mM Tris-HCl pH 7.4, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, and 0.01% SDS) and pre-cleared using Protein A+G magnetic beads (Cat#88802, Thermo Fisher Scientific) at 4◦C for 1 hour. .. Antibodies against H3K36ac (Cat#27683, Cell Signaling Technology) were conjugated to Protein A+G magnetic beads at 4◦C for 1 hour.

    Fluorescence:

    Article Title: Enhancing Antigen Recognition of Living Cells by Site-Specific VHH Antibody Conjugation.
    Article Snippet: .. The fluorescence intensity of the cell lysates, dissolved in 0.25% Triton X-100 in D-PBS(-), was measured using a Thermo Fisher Scientific Varioskan, and cell numbers were calculated from a standard curve (Em: 490 nm, Ex: 515 nm; n= 3). ..



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    a , Immunofluorescence staining of dsDNA (red) and Tom20 (green) in control (sgNTC) and Cpt1a -KO (sgCpt1a) 4T1 cells. Scale bar 20µM. Cytosolic dsDNA coverage was determined as a percent (%) of total area. Unpaired Student’s t-test was performed. b-c , Fractionation of 4T1 control (Ctrl sg or sgNTC) or Cpt1a -KO (Cpt1a sg or sgCpt1a) cells was performed to collect the cytosolic fraction. b , Immunoblot to demonstration fractionation. Markers to denote mitochondrial (Mito., CPT1A and VDAC) and cytosolic (Cyto., cGAS and VINCULIN) fractions are shown. <t>Whole</t> <t>cell</t> lysates (WCL) serve as an input control. c , Relative levels of mitochondrial DNA (mtDNA) regions (Dloop1, Dloop2, mt-16S, mt-Nd4) (left) or nuclear DNA (nDNA, Tert) (right) in the cytosolic fraction was determined using RT-PCR. Unpaired Student’s t-tests were performed. d-e , Control (sgNTC) or Cpt1a -KO (sgCpt1a) 4T1 cells were treated with vehicle (VEH) or dideoxycytidine (ddC) to deplete mtDNA. n=3 per group. d , Relative levels of mtDNA regions (left) or nDNA (right) in the cytosolic fraction was determined using RT-PCR. Two-way ANOVA with Tukey’s post hoc; p=0.0181 (Dloop1), p=0.0109 (mt-16S), p=0.0312 (mt-Nd4), p=0.886 (Tert). e , Relative expression of Ifi44, Irf7, Lcn2 , and Slpi was determined by RT-PCR. Two-way ANOVA with Tukey’s post hoc; p=0.0104 (Ifi44), p=0.00637 (Irf7), p=5.45×10 -4 (Lcn2), p=0.00357 (Slpi). f , Schematic model of mtDNA release from the mitochondrial permeability transition pore (mPTP). CypD acts as regulatory subunit of the mPTP, located within the inner mitochondrial space. VDAC acts as a channel on the outer mitochondrial membrane (OMM). Palmitoylation of CypD at C202 (C203 in humans) prevents CypD from interacting with the mPTP to keep the pore in a closed state. g , Immunoblot of S-palmitoylated (S-pal) CypD in control (sgNTC) and Cpt1a -KO (sgCpt1a) 4T1 cells. Input shows total CPT1A and CypD levels. *p<0.05, **p<0.01, ***p<0.005, ****p<0.001. ns, not significant.
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    a , Immunofluorescence staining of dsDNA (red) and Tom20 (green) in control (sgNTC) and Cpt1a -KO (sgCpt1a) 4T1 cells. Scale bar 20µM. Cytosolic dsDNA coverage was determined as a percent (%) of total area. Unpaired Student’s t-test was performed. b-c , Fractionation of 4T1 control (Ctrl sg or sgNTC) or Cpt1a -KO (Cpt1a sg or sgCpt1a) cells was performed to collect the cytosolic fraction. b , Immunoblot to demonstration fractionation. Markers to denote mitochondrial (Mito., CPT1A and VDAC) and cytosolic (Cyto., cGAS and VINCULIN) fractions are shown. <t>Whole</t> <t>cell</t> lysates (WCL) serve as an input control. c , Relative levels of mitochondrial DNA (mtDNA) regions (Dloop1, Dloop2, mt-16S, mt-Nd4) (left) or nuclear DNA (nDNA, Tert) (right) in the cytosolic fraction was determined using RT-PCR. Unpaired Student’s t-tests were performed. d-e , Control (sgNTC) or Cpt1a -KO (sgCpt1a) 4T1 cells were treated with vehicle (VEH) or dideoxycytidine (ddC) to deplete mtDNA. n=3 per group. d , Relative levels of mtDNA regions (left) or nDNA (right) in the cytosolic fraction was determined using RT-PCR. Two-way ANOVA with Tukey’s post hoc; p=0.0181 (Dloop1), p=0.0109 (mt-16S), p=0.0312 (mt-Nd4), p=0.886 (Tert). e , Relative expression of Ifi44, Irf7, Lcn2 , and Slpi was determined by RT-PCR. Two-way ANOVA with Tukey’s post hoc; p=0.0104 (Ifi44), p=0.00637 (Irf7), p=5.45×10 -4 (Lcn2), p=0.00357 (Slpi). f , Schematic model of mtDNA release from the mitochondrial permeability transition pore (mPTP). CypD acts as regulatory subunit of the mPTP, located within the inner mitochondrial space. VDAC acts as a channel on the outer mitochondrial membrane (OMM). Palmitoylation of CypD at C202 (C203 in humans) prevents CypD from interacting with the mPTP to keep the pore in a closed state. g , Immunoblot of S-palmitoylated (S-pal) CypD in control (sgNTC) and Cpt1a -KO (sgCpt1a) 4T1 cells. Input shows total CPT1A and CypD levels. *p<0.05, **p<0.01, ***p<0.005, ****p<0.001. ns, not significant.
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    Image Search Results


    a , Immunofluorescence staining of dsDNA (red) and Tom20 (green) in control (sgNTC) and Cpt1a -KO (sgCpt1a) 4T1 cells. Scale bar 20µM. Cytosolic dsDNA coverage was determined as a percent (%) of total area. Unpaired Student’s t-test was performed. b-c , Fractionation of 4T1 control (Ctrl sg or sgNTC) or Cpt1a -KO (Cpt1a sg or sgCpt1a) cells was performed to collect the cytosolic fraction. b , Immunoblot to demonstration fractionation. Markers to denote mitochondrial (Mito., CPT1A and VDAC) and cytosolic (Cyto., cGAS and VINCULIN) fractions are shown. Whole cell lysates (WCL) serve as an input control. c , Relative levels of mitochondrial DNA (mtDNA) regions (Dloop1, Dloop2, mt-16S, mt-Nd4) (left) or nuclear DNA (nDNA, Tert) (right) in the cytosolic fraction was determined using RT-PCR. Unpaired Student’s t-tests were performed. d-e , Control (sgNTC) or Cpt1a -KO (sgCpt1a) 4T1 cells were treated with vehicle (VEH) or dideoxycytidine (ddC) to deplete mtDNA. n=3 per group. d , Relative levels of mtDNA regions (left) or nDNA (right) in the cytosolic fraction was determined using RT-PCR. Two-way ANOVA with Tukey’s post hoc; p=0.0181 (Dloop1), p=0.0109 (mt-16S), p=0.0312 (mt-Nd4), p=0.886 (Tert). e , Relative expression of Ifi44, Irf7, Lcn2 , and Slpi was determined by RT-PCR. Two-way ANOVA with Tukey’s post hoc; p=0.0104 (Ifi44), p=0.00637 (Irf7), p=5.45×10 -4 (Lcn2), p=0.00357 (Slpi). f , Schematic model of mtDNA release from the mitochondrial permeability transition pore (mPTP). CypD acts as regulatory subunit of the mPTP, located within the inner mitochondrial space. VDAC acts as a channel on the outer mitochondrial membrane (OMM). Palmitoylation of CypD at C202 (C203 in humans) prevents CypD from interacting with the mPTP to keep the pore in a closed state. g , Immunoblot of S-palmitoylated (S-pal) CypD in control (sgNTC) and Cpt1a -KO (sgCpt1a) 4T1 cells. Input shows total CPT1A and CypD levels. *p<0.05, **p<0.01, ***p<0.005, ****p<0.001. ns, not significant.

    Journal: bioRxiv

    Article Title: CPT1A loss promotes lung metastasis in immune-competent mice via a mechanism of mtDNA release and chronic activation of STING pathway

    doi: 10.64898/2026.05.01.722261

    Figure Lengend Snippet: a , Immunofluorescence staining of dsDNA (red) and Tom20 (green) in control (sgNTC) and Cpt1a -KO (sgCpt1a) 4T1 cells. Scale bar 20µM. Cytosolic dsDNA coverage was determined as a percent (%) of total area. Unpaired Student’s t-test was performed. b-c , Fractionation of 4T1 control (Ctrl sg or sgNTC) or Cpt1a -KO (Cpt1a sg or sgCpt1a) cells was performed to collect the cytosolic fraction. b , Immunoblot to demonstration fractionation. Markers to denote mitochondrial (Mito., CPT1A and VDAC) and cytosolic (Cyto., cGAS and VINCULIN) fractions are shown. Whole cell lysates (WCL) serve as an input control. c , Relative levels of mitochondrial DNA (mtDNA) regions (Dloop1, Dloop2, mt-16S, mt-Nd4) (left) or nuclear DNA (nDNA, Tert) (right) in the cytosolic fraction was determined using RT-PCR. Unpaired Student’s t-tests were performed. d-e , Control (sgNTC) or Cpt1a -KO (sgCpt1a) 4T1 cells were treated with vehicle (VEH) or dideoxycytidine (ddC) to deplete mtDNA. n=3 per group. d , Relative levels of mtDNA regions (left) or nDNA (right) in the cytosolic fraction was determined using RT-PCR. Two-way ANOVA with Tukey’s post hoc; p=0.0181 (Dloop1), p=0.0109 (mt-16S), p=0.0312 (mt-Nd4), p=0.886 (Tert). e , Relative expression of Ifi44, Irf7, Lcn2 , and Slpi was determined by RT-PCR. Two-way ANOVA with Tukey’s post hoc; p=0.0104 (Ifi44), p=0.00637 (Irf7), p=5.45×10 -4 (Lcn2), p=0.00357 (Slpi). f , Schematic model of mtDNA release from the mitochondrial permeability transition pore (mPTP). CypD acts as regulatory subunit of the mPTP, located within the inner mitochondrial space. VDAC acts as a channel on the outer mitochondrial membrane (OMM). Palmitoylation of CypD at C202 (C203 in humans) prevents CypD from interacting with the mPTP to keep the pore in a closed state. g , Immunoblot of S-palmitoylated (S-pal) CypD in control (sgNTC) and Cpt1a -KO (sgCpt1a) 4T1 cells. Input shows total CPT1A and CypD levels. *p<0.05, **p<0.01, ***p<0.005, ****p<0.001. ns, not significant.

    Article Snippet: For mitochondrial DNA (mtDNA) quantification, DNA was isolated from whole-cell lysates, cytosolic fractions, or mitochondria-enriched fractions using the QIAamp DNA Mini Kit (Qiagen #51304).

    Techniques: Immunofluorescence, Staining, Control, Fractionation, Western Blot, Reverse Transcription Polymerase Chain Reaction, Expressing, Permeability, Membrane